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Glutathione

Master Antioxidant | Tripeptide | GSH

Glutathione (GSH) is a tripeptide composed of glutamate, cysteine, and glycine, often called the body's 'master antioxidant.' Found in virtually every cell, it plays critical roles in neutralizing free radicals, detoxifying harmful substances, recycling other antioxidants (vitamins C and E), and supporting immune function. Glutathione levels decline with age and are depleted by stress, toxins, and certain diseases. Injectable forms bypass poor oral bioavailability to deliver glutathione directly to tissues.

Daily dose

200-600 mg (injectable)

Frequency

1-3x per week

Cycle length

4-8 weeks

Storage

Refrigerated (2-8°C)

Key benefits

Master antioxidant neutralizing free radicals. Essential for liver detoxification (Phase II conjugation). Supports immune function and T-cell activity. Recycles vitamins C and E.

How it works

Glutathione's sulfhydryl group donates electrons to neutralize reactive oxygen species. Conjugates toxins via glutathione S-transferase for excretion. Essential for lymphocyte proliferation and optimal immune response. The unique gamma peptide bond resists degradation by peptidases.

Dosage protocols

Goal

General support

Dose

200-400 mg · 1-2x weekly

Route

IM or IV

Goal

Detoxification

Dose

400-600 mg · 2-3x weekly

Route

IV

Goal

Neurological (research)

Dose

1400 mg · 3x weekly

Route

IV infusion

Research indications

antioxidant Support

Master AntioxidantPrimary intracellular antioxidant found in virtually every cell. Neutralizes free radicals and reactive oxygen species.
Antioxidant RecyclingRegenerates oxidized vitamins C and E, extending their protective capacity.

detoxification

Phase II Liver SupportConjugates toxins, drugs, and heavy metals to water-soluble forms for excretion via bile and urine.
Heavy Metal BindingBinds mercury, lead, arsenic, and cadmium for elimination.

immune Function

Lymphocyte SupportEssential for T-cell proliferation and NK cell activity. Even moderate GSH changes profoundly affect immune function.

neurological Health

Parkinson's ResearchGSH is depleted in PD patients' substantia nigra. Clinical trials of IV and intranasal delivery show potential benefits.

Administration

injectable
oral
nasal
topical

Safety notes

Generally well-tolerated

Injection site stinging/pain common if pH not properly buffered (should be pH 6.0-7.0)

IV carries anaphylaxis risk - clinical supervision recommended

Avoid during chemotherapy without oncologist approval

May worsen asthma symptoms

Avoid in pregnancy/breastfeeding - safety not established

Long-term high-dose use may deplete zinc

Research studies

High-dose IV Glutathione Pharmacokinetics - Aebi et al.

Human | 2g/m² IV infusion | Rapid clearance | t½ ~14 minutes

Classic pharmacokinetic study demonstrating that IV glutathione significantly increases plasma levels but has a short half-life (~14 minutes), with rapid conversion to cysteine and other metabolites.

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Glutathione in Parkinson's Disease - Substantia Nigra Depletion

Human | Post-mortem | 40% reduction in substantia nigra

Landmark study showing GSH levels were reduced by 40% in the substantia nigra of Parkinson's patients compared to controls, establishing the oxidative stress connection in PD pathogenesis.

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Intranasal Glutathione Phase IIb Trial - Mischley et al.

Human | 45 PD patients | 3 months | Intranasal GSH

Double-blind, placebo-controlled trial found intranasal glutathione increased brain GSH levels and showed mild motor improvement in Parkinson's patients, though placebo group also improved.

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Glutathione for Skin Lightening - Systematic Review (2025)

Review | Multiple RCTs | Oral, IV, topical forms

Comprehensive review found limited evidence supporting glutathione for skin lightening. Oral and topical forms showed modest effects in some trials, while IV carries safety concerns including hepatotoxicity.

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Glutathione and Immune Function - Droge & Breitkreutz

Review | Lymphocyte function | GSH depletion effects

Demonstrated that even moderate changes in intracellular glutathione have profound effects on lymphocyte function, including T-cell proliferation and NK cell activity.

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GSH in Liver Detoxification - Phase II Conjugation

Review | Hepatic metabolism | Xenobiotic detoxification

Established glutathione's central role in Phase II liver detoxification, conjugating toxins and drugs to water-soluble forms for excretion via bile and urine.

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